Direct Reprogramming of Fibroblasts into Embryonic Sertoli-like Cells by Defined Factors
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Buganim-2012-Direct Reprogramming.pdf
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Author(s)
Buganim, Yosef
Itskovich, Elena
Hu, Yueh-Chiang
Ganz, Kibibi
Sarkar, Sovan
Fu, Dongdong
Welstead, G. Grant
Jaenisch, Rudolf
Cheng, Albert Wu
Page, David C
Date Issued
September 2012
Journal
Cell Stem Cell
Publisher
Elsevier
Citation
Buganim, Yosef, Elena Itskovich, Yueh-Chiang Hu, Albert W. Cheng, Kibibi Ganz, Sovan Sarkar, Dongdong Fu, G. Grant Welstead, David C. Page, and Rudolf Jaenisch. “Direct Reprogramming of Fibroblasts into Embryonic Sertoli-Like Cells by Defined Factors.” Cell Stem Cell 11, no. 3 (September 2012): 373–386. © 2012 Elsevier Inc.
Version
Final published version
Abstract
Sertoli cells are considered the “supporting cells” of the testis that play an essential role in sex determination during embryogenesis and in spermatogenesis during adulthood. Their essential roles in male fertility along with their immunosuppressive and neurotrophic properties make them an attractive cell type for therapeutic applications. Here we demonstrate the generation of induced embryonic Sertoli-like cells (ieSCs) by ectopic expression of five transcription factors. We characterize the role of specific transcription factor combinations in the transition from fibroblasts to ieSCs and identify key steps in the process. Initially, transduced fibroblasts underwent a mesenchymal to epithelial transition and then acquired the ability to aggregate, formed tubular-like structures, and expressed embryonic Sertoli-specific markers. These Sertoli-like cells facilitated neuronal differentiation and self-renewal of neural progenitor cells (NPCs), supported the survival of germ cells in culture, and cooperated with endogenous embryonic Sertoli and primordial germ cells in the generation of testicular cords in the fetal gonad.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1016/j.stem.2012.07.019
