Neural stem cell delivery via porous collagen scaffolds promotes neuronal differentiation and locomotion recovery in spinal cord injury
Name
s41536-020-0097-0.pdf
Description
Published version
Size
7.66 MB
Format
Adobe PDF
Checksum (MD5)
434c2df95bf8277cdb9ff9ba82cdb3c4
Author(s)
Kourgiantaki, Alexandra
Tzeranis, Dimitrios
Karali, Kanelina
Georgelou, Konstantina
Bampoula, Efstathia
Psilodimitrakopoulos, Sotirios
Yannas, Ioannis V
Stratakis, Emmanuel
Sidiropoulou, Kyriaki
Charalampopoulos, Ioannis
Date Issued
June 2020
Journal
npj Regenerative Medicine
Publisher
Springer Science and Business Media LLC
Citation
Kourgiantaki, Alexandra et al. "Neural stem cell delivery via porous collagen scaffolds promotes neuronal differentiation and locomotion recovery in spinal cord injury." npj Regenerative Medicine 5, 1 (June 2020): 12 © 2020 The Author(s)
Version
Final published version
Abstract
Neural stem cell (NSC) grafts have demonstrated significant effects in animal models of spinal cord injury (SCI), yet their clinical translation remains challenging. Significant evidence suggests that the supporting matrix of NSC grafts has a crucial role in regulating NSC effects. Here we demonstrate that grafts based on porous collagen-based scaffolds (PCSs), similar to biomaterials utilized clinically in induced regeneration, can deliver and protect embryonic NSCs at SCI sites, leading to significant improvement in locomotion recovery in an experimental mouse SCI model, so that 12 weeks post-injury locomotion performance of implanted animals does not statistically differ from that of uninjured control animals. NSC-seeded PCS grafts can modulate key processes required to induce regeneration in SCI lesions including enhancing NSC neuronal differentiation and functional integration in vivo, enabling robust axonal elongation, and reducing astrogliosis. Our findings suggest that the efficacy and translational potential of emerging NSC-based SCI therapies could be enhanced by delivering NSC via scaffolds derived from well-characterized clinically proven PCS.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
Terms of Use
Creative Commons Attribution 4.0 International license
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DOI of Published Version
https://doi.org/10.1038/s41536-020-0097-0
