Pooled genetic screens with image‐based profiling
Name
Molecular Systems Biology - 2022 - Walton - Pooled genetic screens with image‐based profiling.pdf
Description
Published version
Size
1.03 MB
Format
Adobe PDF
Checksum (MD5)
8c63f7af19e49c24d0ce06bbe3772086
Author(s)
Walton, Russell T
Singh, Avtar
Blainey, Paul C
Date Issued
November 2022
Journal
Molecular Systems Biology
Publisher
EMBO
Citation
Walton, Russell T, Singh, Avtar and Blainey, Paul C. 2022. "Pooled genetic screens with image‐based profiling." Molecular Systems Biology, 18 (11).
Version
Final published version
Abstract
Spatial structure in biology, spanning molecular, organellular, cellular, tissue, and organismal scales, is encoded through a combination of genetic and epigenetic factors in individual cells. Microscopy remains the most direct approach to exploring the intricate spatial complexity defining biological systems and the structured dynamic responses of these systems to perturbations. Genetic screens with deep single-cell profiling via image features or gene expression programs have the capacity to show how biological systems work in detail by cataloging many cellular phenotypes with one experimental assay. Microscopy-based cellular profiling provides information complementary to next-generation sequencing (NGS) profiling and has only recently become compatible with large-scale genetic screens. Optical screening now offers the scale needed for systematic characterization and is poised for further scale-up. We discuss how these methodologies, together with emerging technologies for genetic perturbation and microscopy-based multiplexed molecular phenotyping, are powering new approaches to reveal genotype-phenotype relationships.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Terms of Use
Creative Commons Attribution 4.0 International license
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.15252/msb.202110768
