Dynamic and regulated TAF gene expression during mouse embryonic germ cell development
Name
journal.pgen.1008515.pdf
Description
Published version
Size
4.26 MB
Format
Adobe PDF
Checksum (MD5)
2e6b96d17b65e81c28b0ee82703a1954
Author(s)
Gura, Megan A.
Mikedis, Maria M.
Seymour, Kimberly A.
de Rooij, Dirk G.
Page, David C
Freiman, Richard N.
Date Issued
January 2020
Journal
PLoS genetics
Publisher
Public Library of Science (PLoS)
Citation
Gura, Megan A. et al. "Dynamic and regulated TAF gene expression during mouse embryonic germ cell development." PLoS Genetics 16, 1 (January 2020): e1008515 © 2020 Gura et al
Version
Final published version
Abstract
Germ cells undergo many developmental transitions before ultimately becoming either eggs or sperm, and during embryonic development these transitions include epigenetic reprogramming, quiescence, and meiosis. To begin understanding the transcriptional regulation underlying these complex processes, we examined the spatial and temporal expression of TAF4b, a variant TFIID subunit required for fertility, during embryonic germ cell development. By analyzing published datasets and using our own experimental system to validate these expression studies, we determined that both Taf4b mRNA and protein are highly germ cell-enriched and that Taf4b mRNA levels dramatically increase from embryonic day 12.5-18.5. Surprisingly, additional mRNAs encoding other TFIID subunits are coordinately upregulated through this time course, including Taf7l and Taf9b. The expression of several of these germ cell-enriched TFIID genes is dependent upon Dazl and/or Stra8, known regulators of germ cell development and meiosis. Together, these data suggest that germ cells employ a highly specialized and dynamic form of TFIID to drive the transcriptional programs that underlie mammalian germ cell development.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution 4.0 International license
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1371/journal.pgen.1008515
