UV Embossed Plastic Chip for Protein Separation and Identification
Name
IMST032.pdf
Size
306.23 KB
Format
Adobe PDF
Checksum (MD5)
5a02b3c11ced873a971d7b1320967006
Author(s)
Guo, Xun
Chan-Park, Mary Bee-Eng
Yoon, Soon Fatt
Chun, Jung-Hoon
Hua, Lin
Sze, Newman
Date Issued
January 2005
Series/Report no.
Innovation in Manufacturing Systems and Technology (IMST);
Abstract
This report demonstrates a UV-embossed polymeric chip for protein separation and identification by Capillary Isoelectric Focusing (CIEF) and Matrix Assisted Laser Desportion/Ionization Mass Spectrometry (MALDI-MS). The polymeric chip has been fabricated by UV-embossing technique with high throughput; the issues in the fabrication have been addressed. In order to achieve high sensitivity of mass detection, five different types of UV curable polymer have been used as sample support to perform protein ionization in Mass Spectrometry (MS); the best results is compared to PMMA, which was the commonly used plastic chip for biomolecular separation. Experimental results show that signal from polyester is 12 times better than that of PMMA in terms of detection sensitivity. Finally, polyester chip is utilized to carry out CIEF to separate proteins, followed by MS identification.
Subjects
Capillary isoselectric focusing
MALDI-MS
UV-embossing
Persistent DSpace Link
