Effect of Virulence Factors on the Photodynamic Inactivation of Cryptococcus neoformans
Name
Prates-2013-Effect of Virulence.pdf
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3.04 MB
Format
Adobe PDF
Checksum (MD5)
b143c6de978e5ff5a81f812043969809
Author(s)
Prates, Renato A.
Burgwyn Fuchs, Beth
Mizuno, Kazue
Naqvi, Qurat
Kato, Ilka T.
Ribeiro, Martha S.
Mylonakis, Eleftherios
Tegos, George P.
Hamblin, Michael R.
Date Issued
January 2013
Journal
PLoS ONE
Publisher
Public Library of Science
Citation
Prates, Renato A. et al. “Effect of Virulence Factors on the Photodynamic Inactivation of Cryptococcus Neoformans.” Ed. Yong-Sun Bahn. PLoS ONE 8.1 (2013): e54387.
Version
Final published version
Abstract
Opportunistic fungal pathogens may cause an array of superficial infections or serious invasive infections, especially in immunocompromised patients. Cryptococcus neoformans is a pathogen causing cryptococcosis in HIV/AIDS patients, but treatment is limited due to the relative lack of potent antifungal agents. Photodynamic inactivation (PDI) uses the combination of non-toxic dyes called photosensitizers and harmless visible light, which produces singlet oxygen and other reactive oxygen species that produce cell inactivation and death. We report the use of five structurally unrelated photosensitizers (methylene blue, Rose Bengal, selenium derivative of a Nile blue dye, a cationic fullerene and a conjugate between poly-L-lysine and chlorin(e6)) combined with appropriate wavelengths of light to inactivate C. neoformans. Mutants lacking capsule and laccase, and culture conditions that favoured melanin production were used to probe the mechanisms of PDI and the effect of virulence factors. The presence of cell wall, laccase and melanin tended to protect against PDI, but the choice of the appropriate photosensitizers and dosimetry was able to overcome this resistance.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
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Creative Commons Attribution
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DOI of Published Version
https://doi.org/10.1371/journal.pone.0054387
