Direct In Vivo Electrochemical Detection of Haemoglobin in Red Blood Cells
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Toh-2014-Direct in vivo.pdf
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1.87 MB
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Author(s)
Toh, Rou Jun
Peng, Weng Kung
Han, Jongyoon
Pumera, Martin
Date Issued
August 2014
Journal
Scientific Reports
Publisher
Nature Publishing Group
Citation
Toh, Rou Jun, Weng Kung Peng, Jongyoon Han, and Martin Pumera. “Direct In Vivo Electrochemical Detection of Haemoglobin in Red Blood Cells.” Sci. Rep. 4 (August 28, 2014): 6209.
Version
Final published version
Abstract
The electrochemical behavior of iron ion in haemoglobin provides insight to the chemical activity in the red blood cell which is important in the field of hematology. Herein, the detection of haemoglobin in human red blood cells on glassy carbon electrode (GC) was demonstrated. Red blood cells or raw blood cells was immobilized on a glassy carbon electrode surface with Nafion films employed to sandwich the layer of biological sample firmly on the electrode surface. Cyclic voltammetry (CV) analyses revealed a well-defined reduction peak for haemoglobin at about −0.30 V (vs. Ag/AgCl) at the red blood cell (GC-Nf-RBC-3Nf) and blood (GC-Nf-B-3Nf) film modified GCE in a pH 3.5 phosphate buffer solution. We further demonstrated that the complex biological conditions of a human red blood cell displayed no interference with the detection of haemoglobin. Such findings shall have an implication on the possibilities of studying the electrochemical behaviour of haemoglobin directly from human blood, for various scientific and clinical purposes.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
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DOI of Published Version
https://doi.org/10.1038/srep06209
