Feasibility of the Assessment of Cholesterol Crystals in Human Macrophages Using Micro Optical Coherence Tomography
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Kashiwagi-2014-Feasibility of the a.pdf
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Author(s)
Kashiwagi, Manabu
Liu, Linbo
Chu, Kengyeh K.
Sun, Chen-Hsin
Tanaka, Atsushi
Gardecki, Joseph A.
Tearney, Guillermo J.
Date Issued
July 2014
Journal
PLoS ONE
Publisher
Public Library of Science
Citation
Kashiwagi, Manabu, Linbo Liu, Kengyeh K. Chu, Chen-Hsin Sun, Atsushi Tanaka, Joseph A. Gardecki, and Guillermo J. Tearney. “Feasibility of the Assessment of Cholesterol Crystals in Human Macrophages Using Micro Optical Coherence Tomography.” Edited by Marc van Zandvoort. PLoS ONE 9, no. 7 (July 21, 2014): e102669.
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Final published version
Abstract
The presence of cholesterol crystals is a hallmark of atherosclerosis, but until recently, such crystals have been considered to be passive components of necrotic plaque cores. Recent studies have demonstrated that phagocytosis of cholesterol crystals by macrophages may actively precipitate plaque progression via an inflammatory pathway, emphasizing the need for methods to study the interaction between macrophages and crystalline cholesterol. In this study, we demonstrate the feasibility of detecting cholesterol in macrophages in situ using Micro-Optical Coherence Tomography (µOCT), an imaging modality we have recently developed with 1-µm resolution. Macrophages containing cholesterol crystals frequently demonstrated highly scattering constituents in their cytoplasm on µOCT imaging, and µOCT was able to evaluate cholesterol crystals in cultured macrophage cells. Our results suggest that µOCT may be useful for the detection and characterization of inflammatory activity associated with cholesterol crystals in the coronary artery.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
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DOI of Published Version
https://doi.org/10.1371/journal.pone.0102669
