Single-Cell Analysis Reveals that Expression of Nanog Is Biallelic and Equally Variable as that of Other Pluripotency Factors in Mouse ESCs
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Jaenisch_Single-cell analysis.pdf
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Author(s)
Wang, Haoyi
Cheng, Albert Wu
Katz, Yarden
Buganim, Yosef
Jaenisch, Rudolf
Faddah, Dina A.
Date Issued
July 2013
Journal
Cell Stem Cell
Publisher
Elsevier
Citation
Faddah, Dina A., Haoyi Wang, Albert Wu Cheng, Yarden Katz, Yosef Buganim, and Rudolf Jaenisch. “Single-Cell Analysis Reveals That Expression of Nanog Is Biallelic and Equally Variable as That of Other Pluripotency Factors in Mouse ESCs.” Cell Stem Cell 13, no. 1 (July 2013): 23–29.
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Author's final manuscript
Abstract
The homeodomain transcription factor Nanog is a central part of the core pluripotency transcriptional network and plays a critical role in embryonic stem cell (ESC) self-renewal. Several reports have suggested that Nanog expression is allelically regulated and that transient downregulation of Nanog in a subset of pluripotent cells predisposes them toward differentiation. Using single-cell gene expression analyses combined with different reporters for the two alleles of Nanog, we show that Nanog is biallelically expressed in ESCs independently of culture condition. We also show that the overall variation in endogenous Nanog expression in ESCs is very similar to that of several other pluripotency markers. Our analysis suggests that reporter-based studies of gene expression in pluripotent cells can be significantly influenced by the gene-targeting strategy and genetic background employed.
MIT Department
Massachusetts Institute of Technology. Computational and Systems Biology Program
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Whitehead Institute for Biomedical Research
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Creative Commons Attribution-NonCommercial-NoDerivs License
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DOI of Published Version
https://doi.org/10.1016/j.stem.2013.04.019
