Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes
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Author(s)
Wang, Ruoxi
MacDuffie, Emily
Santaguida, Stefano
Date Issued
April 2018
Journal
Journal of Visualized Experiments
Publisher
MyJoVE Corporation
Citation
Wang, Ruoxi W., et al. “Generation and Isolation of Cell Cycle-Arrested Cells with Complex Karyotypes.” Journal of Visualized Experiments, no. 134, Apr. 2018. © 2018, Journal of Visualized Experiments.
Version
Final published version
Abstract
Chromosome mis-segregation leads to aneuploidy, a condition in which cells harbor an imbalanced chromosome number. Several lines of evidence strongly indicate that aneuploidy triggers genome instability, ultimately generating cells with complex karyotypes that arrest their proliferation. Isolation and characterization of cells harboring complex karyotypes are crucial to study the impact of an imbalanced chromosome number on cell physiology. To date, no methods have been established to reliably isolate such aneuploid cells. This paper provides a protocol for the enrichment and analysis of aneuploid cells with complex karyotypes utilizing standard, inexpensive tissue culture techniques. This protocol can be used to analyze several features of aneuploid cells with complex karyotypes including their induced senescence-associated secretory phenotype, pro-inflammatory properties, and ability to interact with immune cells. Because cancer cells often harbor imbalances in chromosome number, it is crucial to decipher how aneuploidy impacts cell physiology in normal cells, with the ultimate goal of uncovering both its pro- and anti-tumorigenic effects.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Koch Institute for Integrative Cancer Research at MIT
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.3791/57215
