Role of Era in assembly and homeostasis of the ribosomal small subunit
Name
gkz571.pdf
Description
Published version
Size
10.07 MB
Format
Adobe PDF
Checksum (MD5)
d9575e425512468b5a101baa4827507a
Author(s)
Razi, Aida
Davis, Joseph H
Hao, Yumeng
Jahagirdar, Dushyant
Thurlow, Brett
Basu, Kaustuv
Jain, Nikhil
Gomez-Blanco, Josue
Britton, Robert A
Vargas, Javier
Date Issued
July 2019
Journal
Nucleic Acids Research
Publisher
Oxford University Press (OUP)
Citation
Razi, Aida et al. "Role of Era in assembly and homeostasis of the ribosomal small subunit." Nucleic Acids Research 47, 15 (September 2019): 8301–8317 ©2019, The Author(s).
Version
Final published version
Abstract
Assembly factors provide speed and directionality to the maturation process of the 30S subunit in bacteria. To gain a more precise understanding of how these proteins mediate 30S maturation, it is important to expand on studies of 30S assembly intermediates purified from bacterial strains lacking particular maturation factors. To reveal the role of the essential protein Era in the assembly of the 30S ribosomal subunit, we analyzed assembly intermediates that accumulated in Era-depleted Escherichia coli cells using quantitative mass spectrometry, high resolution cryo-electron microscopy and in-cell footprinting. Our combined approach allowed for visualization of the small subunit as it assembled and revealed that with the exception of key helices in the platform domain, all other 16S rRNA domains fold even in the absence of Era. Notably, the maturing particles did not stall while waiting for the platform domain to mature and instead re-routed their folding pathway to enable concerted maturation of other structural motifs spanning multiple rRNA domains. We also found that binding of Era to the mature 30S subunit destabilized helix 44 and the decoding center preventing binding of YjeQ, another assembly factor. This work establishes Era's role in ribosome assembly and suggests new roles in maintaining ribosome homeostasis.
Subjects
Genetics
MIT Department
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution NonCommercial License 4.0
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1093/nar/gkz571
