Development of a Bioorthogonal and Highly Efficient Conjugation Method for Quantum Dots using Tetrazine-Norbornene Cycloaddition
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Author(s)
Han, Hee-Sun
Devaraj, Neal K.
Lee, Jungmin
Hilderbrand, Scott A.
Weissleder, Ralph
Bawendi, Moungi G.
Date Issued
May 2010
Journal
Journal of the American Chemical Society
Publisher
American Chemical Society
Citation
Han, Hee-Sun et al. “Development of a Bioorthogonal and Highly Efficient Conjugation Method for Quantum Dots Using Tetrazine−Norbornene Cycloaddition.” Journal of the American Chemical Society 132.23 (2010): 7838–7839. Web.
Version
Author's final manuscript
Abstract
We present a bioorthogonal and modular conjugation method for efficient coupling of organic dyes and biomolecules to quantum dots (QDs) using a norbornene−tetrazine cycloaddition. The use of noncoordinating functional groups combined with the rapid rate of the cycloaddition leads to highly efficient conjugation. We have applied this method to the in situ targeting of norbornene-coated QDs to live cancer cells labeled with tetrazine-modified proteins.
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
Massachusetts Institute of Technology. Department of Chemistry
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DOI of Published Version
https://doi.org/10.1021/ja101677r
