A gene expression atlas of embryonic neurogenesis in Drosophila reveals complex spatiotemporal regulation of lncRNAs
Name
dev175265.full.pdf
Description
Published version
Size
14.3 MB
Format
Adobe PDF
Checksum (MD5)
cba3888665b8ef610cfc9f903bcf8ef3
Author(s)
McCorkindale, Alexandra L.
Wahle, Philipp
Werner, Sascha
Jungreis, Irwin
Menzel, Peter
Shukla, Chinmay J.
Abreu, Rúben Lopes Pereira
Irizarry, Rafael A.
Meyer, Irmtraud M.
Kellis, Manolis
Date Issued
March 2019
Journal
Development
Publisher
The Company of Biologists
Citation
McCorkindale, Alexandra L. et al. "A gene expression atlas of embryonic neurogenesis in Drosophila reveals complex spatiotemporal regulation of lncRNAs." Development 146, 6 (March 2019): dev175265 © 2019 Published by The Company of Biologists Ltd
Version
Final published version
Abstract
Cell type specification during early nervous system development in Drosophila melanogaster requires precise regulation of gene expression in time and space. Resolving the programs driving neurogenesis has been a major challenge owing to the complexity and rapidity with which distinct cell populations arise. To resolve the cell type-specific gene expression dynamics in early nervous system development, we have sequenced the transcriptomes of purified neurogenic cell types across consecutive time points covering crucial events in neurogenesis. The resulting gene expression atlas comprises a detailed resource of global transcriptome dynamics that permits systematic analysis of how cells in the nervous system acquire distinct fates. We resolve known gene expression dynamics and uncover novel expression signatures for hundreds of genes among diverse neurogenic cell types, most of which remain unstudied. We also identified a set of conserved long noncoding RNAs (lncRNAs) that are regulated in a tissue-specific manner and exhibit spatiotemporal expression during neurogenesis with exquisite specificity. lncRNA expression is highly dynamic and demarcates specific subpopulations within neurogenic cell types. Our spatiotemporal transcriptome atlas provides a comprehensive resource for investigating the function of coding genes and noncoding RNAs during crucial stages of early neurogenesis.
MIT Department
Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory
Terms of Use
Creative Commons Attribution 4.0 International license
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1242/dev.175265
