Development of a High-Throughput Functional Screen Using Nanowell-Assisted Cell Patterning
Name
Love_Development of a high.pdf
Size
858.51 KB
Format
Adobe PDF
Checksum (MD5)
d1f9b5f3ceb7009169e4e233fd76831d
Author(s)
Ozkumur, Ayca Yalcin
Love, John C
Goods, Brittany A.
Date Issued
September 2015
Journal
Small
Publisher
Wiley Blackwell
Citation
Ozkumur, Ayca Yalcin; Goods, Brittany A. and Love, J. Christopher. “Development of a High-Throughput Functional Screen Using Nanowell-Assisted Cell Patterning.” Small 11, no. 36 (June 2015): 4643–4650 © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Version
Author's final manuscript
Abstract
Living-cell-based screens can facilitate lead discovery of functional therapeutics of interest. A versatile and scalable method is reported that uses dense arrays of nanowells for imparting defined patterns on monolayers of cells. It is shown that this approach can coordinate a multi-component biological assay by designing and implementing a high-throughput, functional nanoliter-scale neutralization assay to identify neutralizing antibodies against HIV.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Chemical Engineering
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1002/smll.201500674
