High-throughput in vivo vertebrate screening
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Yanik_High-throughput.pdf
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Author(s)
Pardo-Martin, Carlos
Chang, Tsung-Yao
Koo, Bryan Kyo
Gilleland, Cody Lee
Wasserman, Steven Charles
Yanik, Mehmet Fatih
Date Issued
July 2010
Journal
Nature Methods
Publisher
Nature Publishing Group
Citation
Pardo-Martin, Carlos et al. “High-throughput in Vivo Vertebrate Screening.” Nature Methods 7.8 (2010): 634–636.
Version
Author's final manuscript
Abstract
We demonstrate a high-throughput platform for cellular-resolution in vivo chemical and genetic screens on zebrafish larvae. The system automatically loads zebrafish from reservoirs or multiwell plates, and positions and rotates them for high-speed confocal imaging and laser manipulation of both superficial and deep organs within 19 s without damage. We performed small-scale test screening of retinal axon guidance mutants and neuronal regeneration assays in combination with femtosecond laser microsurgery.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike 3.0
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DOI of Published Version
https://doi.org/10.1038/nmeth.1481
