Cell cycle regulation during gametogenesis in budding yeast
Name
857790990-MIT.pdf
Description
Full printable version
Size
19.09 MB
Format
Adobe PDF
Checksum (MD5)
857509878c5c121fc14581bef4aab44a
Author(s)
Attner, Michelle Andrea
Advisor(s)
Angelika Amon.
Date Issued
2013
Publisher
Massachusetts Institute of Technology
Abstract
Sexual reproduction depends on meiosis, the specialized cell division that gives rise to gametes. During meiosis, two consecutive rounds of chromosome segregation follow one round of DNA replication to yield four haploid gametes from one diploid progenitor. In meiosis I, homologous chromosomes segregate and in meiosis 11, sister chromatids split. Much of the same cell cycle machinery controls mitosis and meiosis. However, segregation of homologous chromosomes in meiosis I and progression into meiosis 11 directly after meiosis I necessitate several modifications to the basic cell cycle machinery. In this thesis, I have investigated how cell cycle regulators function during gametogenesis. First, I show that the mitotic exit network, which is a signaling pathway essential for mitotic exit, is dispensable for the meiotic divisions, and in fact signals via a mechanism distinct from mitosis. Second, I present data that the Polo kinase Cdc5, which activates mitotic exit in budding yeast, has gained additional roles during meiosis 1. I show that CDC5 is required for the removal of cohesin from chromosome arms in meiosis I, which is a prerequisite for meiosis I segregation. Despite the central role of CDC5 in regulating meiosis I, CDC5 is dispensable during meiosis 11. In sum, understanding how cell cycle regulators control the specialized meiotic divisions has improved our understanding of how different cell division types are established.
Description
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, June 2013.
"June 2013." Cataloged from PDF version of thesis.
Includes bibliographical references.
Subjects
Biology.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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