Noncontact three-dimensional mapping of intracellular hydromechanical properties by Brillouin microscopy
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Kamm_Noncontact three.pdf
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Author(s)
Scarcelli, Giuliano
Patel, Kripa
Polacheck III, William Joseph
Tavakoli Nia, Hadi
Grodzinsky, Alan J
Kamm, Roger Dale
Yun, Seok Hyun (Andy)
Date Issued
October 2015
Journal
Nature Methods
Publisher
Nature Publishing Group
Citation
Scarcelli, Giuliano et al. “Noncontact Three-Dimensional Mapping of Intracellular Hydromechanical Properties by Brillouin Microscopy.” Nature Methods 12.12 (2015): 1132–1134.
Version
Author's final manuscript
Abstract
Current measurements of the biomechanical properties of cells require physical contact with cells or lack subcellular resolution. Here we developed a label-free microscopy technique based on Brillouin light scattering that is capable of measuring an intracellular longitudinal modulus with optical resolution. The 3D Brillouin maps we obtained of cells in 2D and 3D microenvironments revealed mechanical changes due to cytoskeletal modulation and cell-volume regulation.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Massachusetts Institute of Technology. Department of Mechanical Engineering
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1038/nmeth.3616
