Metrology of Individual Small Viruses
Name
Adv Materials Inter - 2023 - Li - Metrology of Individual Small Viruses.pdf
Description
Published version
Size
1.94 MB
Format
Adobe PDF
Checksum (MD5)
9c446b0300df1b9a88991ac4b55cfd40
Author(s)
Li, Kun
Shah, Arjav
Sharma, Rajesh Kumar
Adkins, Raymond
Marjanovic, Tihomir
Doyle, Patrick S
Garaj, Slaven
Date Issued
November 2023
Journal
Advanced Materials Interfaces
Publisher
Wiley
Citation
Li, Kun, Shah, Arjav, Sharma, Rajesh Kumar, Adkins, Raymond, Marjanovic, Tihomir et al. 2023. "Metrology of Individual Small Viruses." Advanced Materials Interfaces, 10 (33).
Version
Final published version
Abstract
Viruses come in various shapes and sizes, and understanding their morphology is central to understanding their activity and function. The need for fast recognition and real‐time fingerprinting methods for pathogenic viruses is a critical bottleneck in implementing many diagnostic and therapeutic techniques. In this work, nanopore tomography (NT) is implemented for fast measurements of the characteristic dimensions of viruses and the optimal operating conditions are explored. Using a small filamentous bacteriophage as a model, it is demonstrated that NT can detect geometrical features in a few‐nanometer regime, with high throughput and accuracy, in aqueous conditions. The instrumental parameters are optimized to obtain virus diameter measurements that are robust to the uncertainties of the external parameters. Furthermore, NT is critically compared to various single‐particle imaging techniques, with a particular emphasis on emerging helium ion microscopy (HIM). It is shown that, with proper operating procedures, HIM can reach a nanometer‐scale resolution in viral metrology, while retaining a high throughput second only to NT. The high throughput of both techniques can foster sufficient statistics for a precise exploration of viral heterogeneity.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Singapore-MIT Alliance in Research and Technology (SMART)
Terms of Use
Creative Commons Attribution
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1002/admi.202300385
