Broader Implications of Defining Standards for the Pluripotency of iPSCs
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Daley-2009-Broader Implications.pdf
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63.83 KB
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Author(s)
Daley, George Q.
Lensch, M. William
Jaenisch, Rudolf
Meissner, Alexander
Plath, Kathrin
Yamanaka, Shinya
Date Issued
March 2009
Journal
Cell Stem Cell
Publisher
Elsevier B.V.
Citation
Daley, George Q., M. William Lensch, Rudolf Jaenisch, Alex Meissner, Kathrin Plath, and Shinya Yamanaka. “Broader Implications of Defining Standards for the Pluripotency of iPSCs.” Cell Stem Cell 4, no. 3 (March 2009): 200–201. © 2009 Elsevier Inc.
Version
Final published version
Abstract
We read with great interest the excellent review by Maherali and Hochedlinger (2008) that recommends standards for characterization of pluripotent stem cell lines, especially the many new lines being generated using factor-based reprogramming techniques (induced pluripotent stem cells, or iPSCs). Of note was the suggestion that iPSCs should be assessed for “functional differentiation through the highest-stringency test acceptable.” For murine iPSCs, this means germline transmission following blastocyst chimerism, and for human iPSCs this means assessment of teratoma pathology. Given the fast pace of discovery in the field, the value and relevance of time-consuming characterization of cell lines are bound to be debated. We'd like to highlight what's at risk when the pressure for rapid publication erodes the imperative for applying rigorous and uniform standards before assigning the label “iPSC” to novel cell lines.
MIT Department
Whitehead Institute for Biomedical Research
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1016/j.stem.2009.02.009
