Efficient CRISPR-Cas9 mediated gene disruption in primary erythroid progenitor cells
Author(s)
Li, Hojun
Shi, Jiahai
Huang, Nai-Jia
Natarajan, Anirudh
Eng, Jennifer C.
Pishesha, Novalia
Lodish, Harvey F
Date Issued
June 2016
Journal
Haematologica
Publisher
European Hematology Association (EHA)/Ferrata Storti Foundation
Citation
Li, H., J. Shi, N.-J. Huang, N. Pishesha, A. Natarajan, J. C. Eng, and H. F. Lodish. “Efficient CRISPR-Cas9 Mediated Gene Disruption in Primary Erythroid Progenitor Cells.” Haematologica 101, no. 6 (March 11, 2016): e216-e219.
Version
Author's final manuscript
Abstract
The study of isolated primary progenitor cells offers great insight into developmental biology and human disease. In particular, ex vivo culture of isolated primary erythroid progenitor cells replicates the differentiation events that occur during in vivo erythropoiesis. Herein we report a high-efficiency method for CRISPR-Cas9 mediated gene disruption in isolated primary erythroid progenitor cells. We use this method to generate the novel result that Lmna is required in terminal erythroid differentiation.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.3324/haematol.2015.135723
