Coupling among growth rate response, metabolic cycle, and cell division cycle in yeast
Name
Slavov-2011-Coupling among growt.pdf
Size
3.43 MB
Format
Adobe PDF
Checksum (MD5)
94818eb74d7ba3d44da516e0a5676d74
Author(s)
Slavov, Nikolai G.
Botstein, David
Date Issued
June 2011
Journal
Molecular Biology of the Cell
Publisher
American Society for Cell Biology
Citation
Slavov, N., and D. Botstein. “Coupling among growth rate response, metabolic cycle, and cell division cycle in yeast.” Molecular Biology of the Cell 22 (2011): 1997-2009.
Version
Final published version
Abstract
We studied the steady-state responses to changes in growth rate of yeast when ethanol is the sole source of carbon and energy. Analysis of these data, together with data from studies where glucose was the carbon source, allowed us to distinguish a “universal” growth rate response (GRR) common to all media studied from a GRR specific to the carbon source. Genes with positive universal GRR include ribosomal, translation, and mitochondrial genes, and those with negative GRR include autophagy, vacuolar, and stress response genes. The carbon source–specific GRR genes control mitochondrial function, peroxisomes, and synthesis of vitamins and cofactors, suggesting this response may reflect the intensity of oxidative metabolism. All genes with universal GRR, which comprise 25% of the genome, are expressed periodically in the yeast metabolic cycle (YMC). We propose that the universal GRR may be accounted for by changes in the relative durations of the YMC phases. This idea is supported by oxygen consumption data from metabolically synchronized cultures with doubling times ranging from 5 to 14 h. We found that the high oxygen consumption phase of the YMC can coincide exactly with the S phase of the cell division cycle, suggesting that oxidative metabolism and DNA replication are not incompatible.
MIT Department
MIT Materials Research Laboratory
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1091/mbc.E11-02-0132
