Millisecond-Timescale Optical Control of Neural Dynamics in the Nonhuman Primate Brain
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Desimone-Millisecond-timescale.pdf
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1.14 MB
Format
Adobe PDF
Checksum (MD5)
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Author(s)
Han, Xue
Qian, Xiaofeng
Bernstein, Jacob G.
Zhou, Huihui
Talei Franzesi, Giovanni
Stern, Patrick
Bronson, Roderick T.
Desimone, Robert
Graybiel, Ann M
Boyden, Edward
Date Issued
April 2009
Journal
Neuron
Publisher
Elsevier B.V.
Citation
Han, Xue et al. “Millisecond-Timescale Optical Control of Neural Dynamics in the Nonhuman Primate Brain.” Neuron 62.2 (2009): 191–198. Web.
Version
Author's final manuscript
Abstract
To understand how brain states and behaviors are generated by neural circuits, it would be useful to be able to perturb precisely the activity of specific cell types and pathways in the nonhuman primate nervous system. We used lentivirus to target the light-activated cation channel channelrhodopsin-2 (ChR2) specifically to excitatory neurons of the macaque frontal cortex. Using a laser-coupled optical fiber in conjunction with a recording microelectrode, we showed that activation of excitatory neurons resulted in well-timed excitatory and suppressive influences on neocortical neural networks. ChR2 was safely expressed, and could mediate optical neuromodulation, in primate neocortex over many months. These findings highlight a methodology for investigating the causal role of specific cell types in nonhuman primate neural computation, cognition, and behavior, and open up the possibility of a new generation of ultraprecise neurological and psychiatric therapeutics via cell-type-specific optical neural control prosthetics.
MIT Department
Massachusetts Institute of Technology. Synthetic Neurobiology Group
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Massachusetts Institute of Technology. Media Laboratory
McGovern Institute for Brain Research at MIT
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike 3.0
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DOI of Published Version
https://doi.org/10.1016/j.neuron.2009.03.011
