Dynamic profiling of the protein life cycle in response to pathogens
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Regev_Dynamic profiling.pdf
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1.01 MB
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Checksum (MD5)
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Author(s)
Jovanovic, M.
Mertins, P.
Przybylski, D.
Chevrier, N.
Satija, R.
Rodriguez, E. H.
Fields, A. P.
Schwartz, S.
Raychowdhury, R.
Mumbach, M. R.
Date Issued
March 2015
Journal
Science
Publisher
American Association for the Advancement of Science (AAAS)
Citation
Jovanovic, M. et al. “Dynamic Profiling of the Protein Life Cycle in Response to Pathogens.” Science 347.6226 (2015): 1259038–1259038.
Version
Author's final manuscript
Abstract
Protein expression is regulated by production and degradation of mRNAs and proteins, but their specific relationships remain unknown. We combine measurements of protein production and degradation and mRNA dynamics to build a quantitative genomic model of the differential regulation of gene expression in LPS-stimulated mouse dendritic cells. Changes in mRNA
abundance play a dominant role in determining most dynamic fold changes in protein levels. Conversely, the preexisting proteome of proteins performing basic cellular functions is remodeled primarily through changes in protein production or degradation, accounting for over half of the
absolute change in protein molecules in the cell. Thus, the proteome is regulated by transcriptional induction of novel cellular functions and remodeling of preexisting functions through the protein life cycle.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Biology
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1126/science.1259038
