Reconstructing and Reprogramming the Tumor-Propagating Potential of Glioblastoma Stem-like Cells
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Regev_Reconstructing and.pdf
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1.65 MB
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Author(s)
Suvà, Mario L.
Rheinbay, Esther
Gillespie, Shawn M.
Patel, Anoop P.
Wakimoto, Hiroaki
Rabkin, Samuel D.
Riggi, Nicolo
Chi, Andrew S.
Cahill, Daniel P.
Nahed, Brian V.
Date Issued
April 2014
Journal
Cell
Publisher
Elsevier
Citation
Suv?, Mario?L. et al. “Reconstructing and Reprogramming the Tumor-Propagating Potential of Glioblastoma Stem-like Cells.” Cell 157.3 (2014): 580–594.
Version
Author's final manuscript
Abstract
Developmental fate decisions are dictated by master transcription factors (TFs) that interact with cis-regulatory elements to direct transcriptional programs. Certain malignant tumors may also depend on cellular hierarchies reminiscent of normal development but superimposed on underlying genetic aberrations. In glioblastoma (GBM), a subset of stem-like tumor-propagating cells (TPCs) appears to drive tumor progression and underlie therapeutic resistance yet remain poorly understood. Here, we identify a core set of neurodevelopmental TFs (POU3F2, SOX2, SALL2, and OLIG2) essential for GBM propagation. These TFs coordinately bind and activate TPC-specific regulatory elements and are sufficient to fully reprogram differentiated GBM cells to “induced” TPCs, recapitulating the epigenetic landscape and phenotype of native TPCs. We reconstruct a network model that highlights critical interactions and identifies candidate therapeutic targets for eliminating TPCs. Our study establishes the epigenetic basis of a developmental hierarchy in GBM, provides detailed insight into underlying gene regulatory programs, and suggests attendant therapeutic strategies.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
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DOI of Published Version
https://doi.org/10.1016/j.cell.2014.02.030
