Two FGFRL-Wnt circuits organize the planarian anteroposterior axis
Name
Scimone-2016-Two FGFRL-Wnt circui.pdf
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5.96 MB
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Author(s)
Scimone, M. Lucila
Cote, Lauren Esther
Reddien, Peter
Rogers, Travis
Date Issued
April 2016
Journal
eLife
Publisher
eLife Sciences Publications, Ltd.
Citation
Scimone, M. Lucila, Lauren E. Cote, Travis Rogers, and Peter W. Reddien. “Two FGFRL-Wnt Circuits Organize the Planarian Anteroposterior Axis.” eLife 5 (April 11, 2016).
Version
Final published version
Abstract
How positional information instructs adult tissue maintenance is poorly understood. Planarians undergo whole-body regeneration and tissue turnover, providing a model for adult positional information studies. Genes encoding secreted and transmembrane components of multiple developmental pathways are predominantly expressed in planarian muscle cells. Several of these genes regulate regional identity, consistent with muscle harboring positional information. Here, single-cell RNA-sequencing of 115 muscle cells from distinct anterior-posterior regions identified 44 regionally expressed genes, including multiple Wnt and ndk/FGF receptor-like (ndl/FGFRL) genes. Two distinct FGFRL-Wnt circuits, involving juxtaposed anterior FGFRL and posterior Wnt expression domains, controlled planarian head and trunk patterning. ndl-3 and wntP-2 inhibition expanded the trunk, forming ectopic mouths and secondary pharynges, which independently extended and ingested food. fz5/8-4 inhibition, like that of ndk and wntA, caused posterior brain expansion and ectopic eye formation. Our results suggest that FGFRL-Wnt circuits operate within a body-wide coordinate system to control adult axial positioning.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
Terms of Use
Creative Commons Attribution 4.0 International License
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DOI of Published Version
https://doi.org/10.7554/eLife.12845
