Diffraction optical tomography using a quantitative phase imaging unit
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Author(s)
Kim, Kyoohyun
Yaqoob, Zahid
Lee, Kyeoreh
Kang, Jeon Woong
Choi, Youngwoon
Hosseini, Poorya
So, Peter T. C.
Park, YongKeun
Date Issued
December 2014
Journal
Optics Letters
Publisher
The Optical Society
Citation
Kim, Kyoohyun, Zahid Yaqoob, KyeoReh Lee, Jeon Woong Kang, Youngwoon Choi, Poorya Hosseini, Peter T. C. So, and YongKeun Park. “Diffraction Optical Tomography Using a Quantitative Phase Imaging Unit.” Optics Letters 39, no. 24 (December 12, 2014): 6935.
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Author's final manuscript
Abstract
A simple and practical method to measure three-dimensional (3-D) refractive index (RI) distributions of biological cells is presented. A common-path self-reference interferometry consisting of a compact set of polarizers is attached to a conventional inverted microscope equipped with a beam scanning unit, which can precisely measure multiple 2-D holograms of a sample with high phase stability for various illumination angles, from which accurate 3-D optical diffraction tomograms of the sample can be reconstructed. 3-D RI tomograms of nonbiological samples such as polystyrene microspheres, as well as biological samples including human red blood cells and breast cancer cells, are presented.
MIT Department
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Chemistry
Massachusetts Institute of Technology. Department of Mechanical Engineering
Massachusetts Institute of Technology. Spectroscopy Laboratory
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DOI of Published Version
https://doi.org/10.1364/OL.39.006935
