Nanometric self-assembling peptide layers maintain adult hepatocyte phenotype in sandwich cultures
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1477-3155-8-29.pdf
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Checksum (MD5)
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Author(s)
Wu, Jonathan
Mari-Buye, Nuria
Fernandez Muinos, Teresa
Borros, Salvador
Favia, Pietro
Semino, Carlos Eduardo
Date Issued
December 2010
Journal
Journal of Nanobiotechnology
Publisher
BioMed Central Ltd
Citation
Wu, Jonathan et al. “Nanometric Self-assembling Peptide Layers Maintain Adult Hepatocyte Phenotype in Sandwich Cultures.” Journal of Nanobiotechnology 8.1 (2010) : 29. © 2010 Wu et al; licensee BioMed Central
Version
Final published version
Abstract
Background: Isolated hepatocytes removed from their microenvironment soon lose their hepatospecific functions when cultured. Normally hepatocytes are commonly maintained under limited culture medium supply as well as scaffold thickness. Thus, the cells are forced into metabolic stress that degenerate liver specific functions. This study aims to improve hepatospecific activity by creating a platform based on classical collagen sandwich cultures. Results: The modified sandwich cultures replace collagen with self-assembling peptide, RAD16-I, combined with functional peptide motifs such as the integrin-binding sequence RGD and the laminin receptor binding sequence YIG to create a cell-instructive scaffold. In this work, we show that a plasma-deposited coating can be used to obtain a peptide layer thickness in the nanometric range, which in combination with the incorporation of functional peptide motifs have a positive effect on the expression of adult hepatocyte markers including albumin, CYP3A2 and HNF4-alpha. Conclusions: This study demonstrates the capacity of sandwich cultures with modified instructive self-assembling peptides to promote cell-matrix interaction and the importance of thinner scaffold layers to overcome mass transfer problems. We believe that this bioengineered platform improves the existing hepatocyte culture methods to be used for predictive toxicology and eventually for hepatic assist technologies and future artificial organs.
MIT Department
Massachusetts Institute of Technology. Center for Biomedical Engineering
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Creative Commons Attribution
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DOI of Published Version
https://doi.org/10.1186/1477-3155-8-29
