Fast multiple gene fragment ligation method based on Type IIs restriction enzyme DraIII
Name
BBFRFC61.pdf
Size
285.52 KB
Format
Adobe PDF
Checksum (MD5)
70a2ae546e102e004c220f546975e6df
Author(s)
Shi, Zhenyu
Li, Teng
Chen, Guoqiang
Date Issued
October 31, 2010
Series/Report no.
BBF RFC;61
Abstract
With the established BioBrick Assembly standards, ligation of different parts has to be accomplished step by step. It can be time-consuming when dealing with multiple fragment ligation. BBF RFC 61 is developed aimed at completing the ligation of multiple fragments quickly and efficiently based on Type II restriction enzyme DraIII.
Subjects
assembly
method
Terms of Use
Attribution-No Derivative Works 3.0 United States
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