Design and testing of the thermal properties of the structure of an ultra high-throughput mutational spectrometer
Name
57615152-MIT.pdf
Description
Full printable version
Size
4.22 MB
Format
Adobe PDF
Checksum (MD5)
429f61c399ecc258d1c7308861d310fa
Author(s)
Del Zio, Michael R. (Michael Robert), 1982-
Advisor(s)
Ian W. Hunter.
Date Issued
2004
Publisher
Massachusetts Institute of Technology
Abstract
A process known as mutational spectrometry allows the detection of both single and multiple mutations that appear to be spontaneous, using a technique known as constant denaturing capillary electrophoresis (CDCE). CDCE requires a region of constant temperature and concentration of denaturant. A massively parallel, fully automated instrument, capable of handling as many as 10,000 DNA samples simultaneously, is suited to this technique. A modular structure of such a mutational spectrometer was designed to remain water-tight, provide an array to hold the capillaries for electrophoretic excitation, and modulate the flow of a heat transfer fluid. Six such modules were manufactured and assembled. As the heat transfer fluid passed through the assembled structure, the natural thermal loss was determined.
Description
Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2004.
Includes bibliographical references (leaves 42-43).
Subjects
Mechanical Engineering.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
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