Objective, comparative assessment of the penetration depth of temporal-focusing microscopy for imaging various organs
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Bawendi_Objective, comparative.pdf
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Author(s)
Rowlands, Christopher
Bruns, Oliver Thomas
Bawendi, Moungi G
So, Peter T. C.
Date Issued
April 2015
Journal
Journal of Biomedical Optics
Publisher
SPIE
Citation
Rowlands, Christopher J.; Bruns, Oliver T.; Bawendi, Moungi G. and So, Peter T. C. "Objective, comparative assessment of the penetration depth of temporal-focusing microscopy for imaging various organs." Journal of Biomedical Optics 20, 6 (April 2015): 061107 © The Authors
Version
Final published version
Abstract
Temporal focusing is a technique for performing axially resolved widefield multiphoton microscopy with a large field of view. Despite significant advantages over conventional point-scanning multiphoton microscopy in terms of imaging speed, the need to collect the whole image simultaneously means that it is expected to achieve a lower penetration depth in common biological samples compared to point-scanning. We assess the penetration depth using a rigorous objective criterion based on the modulation transfer function, comparing it to point-scanning multiphoton microscopy. Measurements are performed in a variety of mouse organs in order to provide practical guidance as to the achievable penetration depth for both imaging techniques. It is found that two-photon scanning microscopy has approximately twice the penetration depth of temporal-focusing microscopy, and that penetration depth is organ-specific; the heart has the lowest penetration depth, followed by the liver, lungs, and kidneys, then the spleen, and finally white adipose tissue.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Chemistry
Massachusetts Institute of Technology. Department of Mechanical Engineering
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1117/1.jbo.20.6.061107
