Microfluidic probe for single-cell lysis and analysis in adherent tissue culture
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Lauffenburger_Microfluidic probe.pdf
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Author(s)
Sarkar, Aniruddh
Kolitz, Sarah
Lauffenburger, Douglas A.
Han, Jongyoon
Alternative Title
Microfluidic probe for single-cell analysis in adherent tissue culture
Date Issued
March 2014
Journal
Nature Communications
Publisher
Nature Publishing Group
Citation
Sarkar, Aniruddh, Sarah Kolitz, Douglas A. Lauffenburger, and Jongyoon Han. “Microfluidic Probe for Single-Cell Analysis in Adherent Tissue Culture.” Nat Comms 5 (March 5, 2014).
Version
Author's final manuscript
Abstract
Single-cell analysis provides information critical to understanding key disease processes that are characterized by significant cellular heterogeneity. Few current methods allow single-cell measurement without removing cells from the context of interest, which not only destroys contextual information but also may perturb the process under study. Here we present a microfluidic probe that lyses single adherent cells from standard tissue culture and captures the contents to perform single-cell biochemical assays. We use this probe to measure kinase and housekeeping protein activities, separately or simultaneously, from single human hepatocellular carcinoma cells in adherent culture. This tool has the valuable ability to perform measurements that clarify connections between extracellular context, signals and responses, especially in cases where only a few cells exhibit a characteristic of interest.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Massachusetts Institute of Technology. Research Laboratory of Electronics
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1038/ncomms4421
