Antibody Data Analysis from Diverse Immune Libraries
Name
nihms-2070484.pdf
Description
Accepted version
Size
336.06 KB
Format
Adobe PDF
Checksum (MD5)
9b2047faa5f1ebef2a119b1c8f009aa4
Author(s)
Fahad, Ahmed S
Gutiérrez-Gonzalez, Matías F
Madan, Bharat
DeKosky, Brandon J
Date Issued
2025
Journal
Cold Spring Harbor Protocols
Publisher
Cold Spring Harbor Laboratory
Version
Author's final manuscript
Abstract
Antibody functional screening studies and next-generation sequencing require careful processing and interpretation of sequence data for optimal results. Here, we provide a detailed protocol for the functional analysis of antibody gene data, including antibody repertoire quantification and functional mapping of high-throughput screening data based on enrichment ratio values, which are a simple way to determine the enrichment of each sequenced antibody after sorting a display library against desired antigens. This protocol enables a user to apply a set of simple yet powerful bioinformatic tools for high-throughput analysis and interpretation of antibody data that is especially well suited for display library screening and for antibody discovery applications.
Terms of Use
Creative Commons Attribution-NonCommercial-ShareAlike
Persistent DSpace Link
DOI of Published Version
https://cshprotocols.cshlp.org/content/2025/1/pdb.prot108627.long
