In situ electrochemical generation of nitric oxide for neuronal modulation
Author(s)
Park, Jimin
Jin, Kyoungsuk
Sahasrabudhe, Atharva
Chiang, Po Han
Maalouf, Joseph H.
Koehler, Florian
Rosenfeld, Dekel
Rao, Siyuan
Tanaka, Tomo
Khudiyev, Tural
Date Issued
June 2020
Journal
Nature Nanotechnology
Publisher
Springer Science and Business Media LLC
Citation
Park, Jimin et al. "In situ electrochemical generation of nitric oxide for neuronal modulation." Nature Nanotechnology (June 2020) © 2020 The Author(s)
Version
Author's final manuscript
Abstract
Understanding the function of nitric oxide, a lipophilic messenger in physiological processes across nervous, cardiovascular and immune systems, is currently impeded by the dearth of tools to deliver this gaseous molecule in situ to specific cells. To address this need, we have developed iron sulfide nanoclusters that catalyse nitric oxide generation from benign sodium nitrite in the presence of modest electric fields. Locally generated nitric oxide activates the nitric oxide-sensitive cation channel, transient receptor potential vanilloid family member 1 (TRPV1), and the latency of TRPV1-mediated Ca2+ responses can be controlled by varying the applied voltage. Integrating these electrocatalytic nanoclusters with multimaterial fibres allows nitric oxide-mediated neuronal interrogation in vivo. The in situ generation of nitric oxide in the ventral tegmental area with the electrocatalytic fibres evoked neuronal excitation in the targeted brain region and its excitatory projections. This nitric oxide generation platform may advance mechanistic studies of the role of nitric oxide in the nervous system and other organs.
MIT Department
Massachusetts Institute of Technology. Department of Materials Science and Engineering
Massachusetts Institute of Technology. Research Laboratory of Electronics
McGovern Institute for Brain Research at MIT
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Department of Chemistry
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies
Simons Center for the Social Brain (Massachusetts Institute of Technology)
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/s41565-020-0701-x
