Automated sequence design of 2D wireframe DNA origami with honeycomb edges
Name
s41467-019-13457-y.pdf
Description
Published version
Size
5.55 MB
Format
Adobe PDF
Checksum (MD5)
1375af99a0982594a03ca167b0fbbbce
Author(s)
Jun, Hyungmin
Wang, Xiao
Bricker, William P
Bathe, Mark
Date Issued
November 2019
Journal
Nature Communications
Publisher
Springer
Citation
Jun, Hyungmin et al. "Automated sequence design of 2D wireframe DNA origami with honeycomb edges." Nature Communications 10, 1 (November 2019): 5419 © 2019 The Author(s)
Version
Final published version
Abstract
Wireframe DNA origami has emerged as a powerful approach to fabricating nearly arbitrary 2D and 3D geometries at the nanometer-scale. Complex scaffold and staple routing needed to design wireframe DNA origami objects, however, render fully automated, geometry-based sequence design approaches essential for their synthesis. And wireframe DNA origami structural fidelity can be limited by wireframe edges that are composed only of one or two duplexes. Here we introduce a fully automated computational approach that programs 2D wireframe origami assemblies using honeycomb edges composed of six parallel duplexes. These wireframe assemblies show enhanced structural fidelity from electron microscopy-based measurement of programmed angles compared with identical geometries programmed using dual-duplex edges. Molecular dynamics provides additional theoretical support for the enhanced structural fidelity observed. Application of our top-down sequence design procedure to a variety of complex objects demonstrates its broad utility for programmable 2D nanoscale materials.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Mechanical Engineering
Terms of Use
Creative Commons Attribution 4.0 International license
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/s41467-019-13457-y
