Fitness advantages conferred by the L20-interacting RNA cis -regulator of ribosomal protein synthesis in Bacillus subtilis
Name
RNA-2018-Babina-1133-43.pdf
Description
Published version
Size
1.17 MB
Format
Adobe PDF
Checksum (MD5)
1d73ad793ef873e2421566e07ceec940
Author(s)
Parker, Darren J.
Li, Gene-Wei
Date Issued
September 2018
Journal
RNA (New York, N.Y.)
Publisher
Cold Spring Harbor Laboratory
Citation
Babina, Arrianne M. et al. “Fitness advantages conferred by the L20-interacting RNA cis -regulator of ribosomal protein synthesis in Bacillus subtilis.” RNA (New York, N.Y.) 24 (2018): 1133-1143 © 2018 The Author(s)
Version
Final published version
Abstract
In many bacteria, ribosomal proteins autogenously repress their own expression by interacting with RNA structures typically located in the 5′′-UTRs of their mRNA transcripts. This regulation is necessary to maintain a balance between ribosomal proteins and rRNA to ensure proper ribosome production. Despite advances in noncoding RNA discovery and validation of RNA-protein regulatory interactions, the selective pressures that govern the formation and maintenance of such RNA cis-regulators in the context of an organism remain largely undetermined. To examine the impact disruptions to this regulation have on bacterial fitness, we introduced point mutations that abolish ribosomal protein binding and regulation into the RNA structure that controls expression of ribosomal proteins L20 and L35 within the Bacillus subtilis genome. Our studies indicate that removing this regulation results in reduced log phase growth, improper rRNA maturation, and the accumulation of a kinetically trapped or misassembled ribosomal particle at low temperatures, suggesting defects in ribosome synthesis. Such work emphasizes the important role regulatory RNAs play in the stoichiometric production of ribosomal components for proper ribosome composition and overall organism viability and reinforces the potential of targeting ribosomal protein production and ribosome assembly with novel antimicrobials.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution 4.0 International license
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1261/RNA.065011.117
