A Portable Chemotaxis Platform for Short and Long Term Analysis
Name
Xu-2012-A Portable Chemotaxi.pdf
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2.11 MB
Format
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Checksum (MD5)
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Author(s)
Xu, Chenjie
Poh, Yuk Kee C.
Roes, Isaac Victor
O'Cearbhaill, Eoin D.
Matthiesen, Mads Emil
Mu, Luye
Yang, Seung Yun
Miranda-Nieves, David
Irimia, Daniel
Karp, Jeffrey Michael
Date Issued
September 2012
Journal
PLoS One
Publisher
Public Library of Science
Citation
Xu, Chenjie et al. “A Portable Chemotaxis Platform for Short and Long Term Analysis.” Ed. Jianghong Rao. PLoS ONE 7.9 (2012): e44995. Web.
Version
Final published version
Abstract
Flow-based microfluidic systems have been widely utilized for cell migration studies given their ability to generate versatile and precisely defined chemical gradients and to permit direct visualization of migrating cells. Nonetheless, the general need for bulky peripherals such as mechanical pumps and tubing and the complicated setup procedures significantly limit the widespread use of these microfluidic systems for cell migration studies. Here we present a simple method to power microfluidic devices for chemotaxis assays using the commercially available ALZET® osmotic pumps. Specifically, we developed a standalone chemotaxis platform that has the same footprint as a multiwell plate and can generate well-defined, stable chemical gradients continuously for up to 7 days. Using this platform, we validated the short-term (24 hours) and long-term (72 hours) concentration dependent PDGF-BB chemotaxis response of human bone marrow derived mesenchymal stem cells.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Department of Materials Science and Engineering
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Creative Commons Attribution
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DOI of Published Version
https://doi.org/10.1371/journal.pone.0044995
