Engineering alternative butanol production platforms in heterologous bacteria
Name
Prather-Engineering Alternative Butanol.pdf
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914.21 KB
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Author(s)
Nielsen, David R.
Leonard, Effendi
Yoon, Sang-Hwal
Tseng, Hsien-chung
Yuan, Clara J.
Prather, Kristala L. Jones
Date Issued
May 2009
Journal
Metabolic Engineering
Publisher
Elsevier B.V.
Citation
Nielsen, David R. et al. “Engineering alternative butanol production platforms in heterologous bacteria.” Metabolic Engineering 11.4-5 (2009): 262-273.
Version
Author's final manuscript
Abstract
Alternative microbial hosts have been engineered as biocatalysts for butanol biosynthesis. The butanol synthetic pathway of Clostridium acetobutylicum was first re-constructed in Escherichia coli to establish a baseline for comparison to other hosts. Whereas polycistronic expression of the pathway genes resulted in the production of 34 mg/L butanol, individual expression of pathway genes elevated titers to 200 mg/L. Improved titers were achieved by co-expression of Saccharomyces cerevisiae formate dehydrogenase while overexpression of E. coli glyceraldehyde 3-phosphate dehydrogenase to elevate glycolytic flux improved titers to 580 mg/L. Pseudomonas putida and Bacillus subtilis were also explored as alternative production hosts. Polycistronic expression of butanol biosynthetic genes yielded butanol titers of 120 and 24 mg/L from P. putida and B. subtilis, respectively. Production in the obligate aerobe P. putida was dependent upon expression of bcd-etfAB. These results demonstrate the potential of engineering butanol biosynthesis in a variety of heterologous microorganisms, including those cultivated aerobically.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike 3.0
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DOI of Published Version
https://doi.org/10.1016/j.ymben.2009.05.003
