Optical Diffraction Tomography and Raman Confocal Microscopy for the Investigation of Vacuoles Associated with Cancer Senescent Engulfing Cells
Name
biosensors-13-00973-v2.pdf
Size
5.54 MB
Format
Adobe PDF
Checksum (MD5)
7c8f7351888ec6d430d74ad5f2cac35e
Author(s)
Ghislanzoni, Silvia
Kang, Jeon Woong
Bresci, Arianna
Masella, Andrea
Kobayashi-Kirschvink, Koseki J.
Polli, Dario
Bongarzone, Italia
So, Peter T. C.
Date Issued
November 7, 2023
Publisher
Multidisciplinary Digital Publishing Institute
Citation
Biosensors 13 (11): 973 (2023)
Version
Final published version
Abstract
Wild-type p53 cancer therapy-induced senescent cells frequently engulf and degrade neighboring ones inside a massive vacuole in their cytoplasm. After clearance of the internalized cell, the vacuole persists, seemingly empty, for several hours. Despite large vacuoles being associated with cell death, this process is known to confer a survival advantage to cancer engulfing cells, leading to therapy resistance and tumor relapse. Previous attempts to resolve the vacuolar structure and visualize their content using dyes were unsatisfying for lack of known targets and ineffective dye penetration and/or retention. Here, we overcame this problem by applying optical diffraction tomography and Raman spectroscopy to MCF7 doxorubicin-induced engulfing cells. We demonstrated a real ability of cell tomography and Raman to phenotype complex microstructures, such as cell-in-cells and vacuoles, and detect chemical species in extremely low concentrations within live cells in a completely label-free fashion. We show that vacuoles had a density indistinguishable to the medium, but were not empty, instead contained diluted cell-derived macromolecules, and we could discern vacuoles from medium and cells using their Raman fingerprint. Our approach is useful for the noninvasive investigation of senescent engulfing (and other peculiar) cells in unperturbed conditions, crucial for a better understanding of complex biological processes.
MIT Department
Massachusetts Institute of Technology. Laser Biomedical Research Center
Terms of Use
Creative Commons Attribution
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.3390/bios13110973
