Carbon-Nanotube-Embedded Hydrogel Sheets for Engineering Cardiac Constructs and Bioactuators
Name
Kong_Carbon-nanotube.pdf
Size
3.27 MB
Format
Adobe PDF
Checksum (MD5)
fd13ace31680fa8b3c270064098f6712
Author(s)
Shin, Su Ryon
Jung, Sung Mi
Zalabany, Momen
Kim, Keekyoung
Zorlutuna, Pinar
Kim, Sang bok
Nikkhah, Mehdi
Khabiry, Masoud
Azize, Mohamed
Kong, Jing
Date Issued
March 2013
Journal
ACS Nano
Publisher
American Chemical Society
Citation
Shin, Su Ryon, Sung Mi Jung, Momen Zalabany, Keekyoung Kim, Pinar Zorlutuna, Sang bok Kim, Mehdi Nikkhah, et al. “Carbon-Nanotube-Embedded Hydrogel Sheets for Engineering Cardiac Constructs and Bioactuators.” ACS Nano 7, no. 3 (March 26, 2013): 2369–2380.
Version
Author's final manuscript
Abstract
We engineered functional cardiac patches by seeding neonatal rat cardiomyocytes onto carbon nanotube (CNT)-incorporated photo-cross-linkable gelatin methacrylate (GelMA) hydrogels. The resulting cardiac constructs showed excellent mechanical integrity and advanced electrophysiological functions. Specifically, myocardial tissues cultured on 50 μm thick CNT-GelMA showed 3 times higher spontaneous synchronous beating rates and 85% lower excitation threshold, compared to those cultured on pristine GelMA hydrogels. Our results indicate that the electrically conductive and nanofibrous networks formed by CNTs within a porous gelatin framework are the key characteristics of CNT-GelMA leading to improved cardiac cell adhesion, organization, and cell–cell coupling. Centimeter-scale patches were released from glass substrates to form 3D biohybrid actuators, which showed controllable linear cyclic contraction/extension, pumping, and swimming actuations. In addition, we demonstrate for the first time that cardiac tissues cultured on CNT-GelMA resist damage by a model cardiac inhibitor as well as a cytotoxic compound. Therefore, incorporation of CNTs into gelatin, and potentially other biomaterials, could be useful in creating multifunctional cardiac scaffolds for both therapeutic purposes and in vitro studies. These hybrid materials could also be used for neuron and other muscle cells to create tissue constructs with improved organization, electroactivity, and mechanical integrity.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Research Laboratory of Electronics
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1021/nn305559j
