Linking single-cell measurements of mass, growth rate, and gene expression
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13059_2018_Article_1576.pdf
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Author(s)
Pelton, Kristine
De Smet, Frederik
Ligon, Keith L.
Kimmerling, Robert John
Prakadan, Sanjay
Gupta, Alejandro J.
Calistri, Nicholas L
Stevens, Mark M.
Olcum, Selim A.
Cermak, Nathan
Date Issued
November 2018
Journal
Genome Biology
Publisher
BioMed Central
Citation
Kimmerling, Robert J., et al. “Linking Single-Cell Measurements of Mass, Growth Rate, and Gene Expression.” Genome Biology, vol. 19, no. 1, Dec. 2018. © 2018 The Authors
Version
Final published version
Abstract
Mass and growth rate are highly integrative measures of cell physiology not discernable via genomic measurements. Here, we introduce a microfluidic platform enabling direct measurement of single-cell mass and growth rate upstream of highly multiplexed single-cell profiling such as single-cell RNA sequencing. We resolve transcriptional signatures associated with single-cell mass and growth rate in L1210 and FL5.12 cell lines and activated CD8+ T cells. Further, we demonstrate a framework using these linked measurements to characterize biophysical heterogeneity in a patient-derived glioblastoma cell line with and without drug treatment. Our results highlight the value of coupled phenotypic metrics in guiding single-cell genomics. Keywords: Single-cell RNA-Seq, Mass, Growth, Serial suspended microchannel resonator, Multi-omics, Single cell, T cell activation, Glioblastoma, GBM, Drug response, Microfluidics, Biophysical properties
MIT Department
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Chemistry
Koch Institute for Integrative Cancer Research at MIT
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Creative Commons Attribution
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DOI of Published Version
https://doi.org/10.1186/s13059-018-1576-0
