Defining early steps in Bacillus subtilis biofilm biosynthesis
Name
arbour-et-al-2023-defining-early-steps-in-bacillus-subtilis-biofilm-biosynthesis.pdf
Description
Published version
Size
2.15 MB
Format
Adobe PDF
Checksum (MD5)
850444ddd32b86679ce8570f8f1ca675
Author(s)
Arbour, Christine A
Nagar, Rupa
Bernstein, Hannah M
Ghosh, Soumi
Al-Sammarraie, Yusra
Dorfmueller, Helge C
Ferguson, Michael AJ
Stanley-Wall, Nicola R
Imperiali, Barbara
Date Issued
August 31, 2023
Journal
mBio
Publisher
American Society for Microbiology
Citation
Arbour CA, Nagar R, Bernstein HM, Ghosh S, Al-Sammarraie Y, Dorfmueller HC, Ferguson MAJ, Stanley-Wall NR, Imperiali B. 2023. Defining early steps in Bacillus subtilis biofilm biosynthesis. mBio 14:e00948-23.
Version
Final published version
Abstract
The Bacillus subtilis extracellular biofilm matrix includes an exopolysaccharide (EPS) that is critical for the architecture and function of the community. To date, our understanding of the biosynthetic machinery and the molecular composition of the EPS of B. subtilis remains unclear and incomplete. This report presents synergistic biochemical and genetic studies built from a foundation of comparative sequence analyses targeted at elucidating the activities of the first two membrane-committed steps in the EPS biosynthetic pathway. By taking this approach, we determined the nucleotide sugar donor and lipid-linked acceptor substrates for the first two enzymes in the B. subtilis biofilm EPS biosynthetic pathway. EpsL catalyzes the first phosphoglycosyl transferase step using uridine diphosphate (UDP)-di-N-acetyl bacillosamine as phospho-sugar donor. EpsD is a predicted GT-B fold (GT4 family) retaining glycosyl transferase that catalyzes the second step in the pathway that utilizes the product of EpsL as an acceptor substrate and UDP-N-acetyl glucosamine as the sugar donor. Thus, the study defines the first two monosaccharides at the reducing end of the growing EPS unit. In doing so, we provide the first evidence of the presence of bacillosamine in an EPS synthesized by a Gram-positive bacterium.
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DOI of Published Version
https://doi.org/10.1128/mbio.00948-23
