Deletion of VPS50 protein in mouse brain impairs synaptic function and behavior
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12915_2024_Article_1940.pdf
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Author(s)
Ahumada-Marchant, Constanza
Ancatén-Gonzalez, Carlos
Haensgen, Henny
Brauer, Bastian
Merino-Veliz, Nicolas
Droste, Rita
Arancibia, Felipe
Horvitz, H. Robert
Constantine-Paton, Martha
Arriagada, Gloria
Date Issued
June 26, 2024
Journal
BMC Biology
Publisher
Springer Science and Business Media LLC
Citation
Ahumada-Marchant, C., Ancatén-Gonzalez, C., Haensgen, H. et al. Deletion of VPS50 protein in mouse brain impairs synaptic function and behavior. BMC Biol 22, 142 (2024).
Version
Final published version
Abstract
Background
The VPS50 protein functions in synaptic and dense core vesicle acidification, and perturbations of VPS50 function produce behavioral changes in Caenorhabditis elegans. Patients with mutations in VPS50 show severe developmental delay and intellectual disability, characteristics that have been associated with autism spectrum disorders (ASDs). The mechanisms that link VPS50 mutations to ASD are unknown.
Results
To examine the role of VPS50 in mammalian brain function and behavior, we used the CRISPR/Cas9 system to generate knockouts of VPS50 in both cultured murine cortical neurons and living mice. In cultured neurons, KO of VPS50 did not affect the number of synaptic vesicles but did cause mislocalization of the V-ATPase V1 domain pump and impaired synaptic activity, likely as a consequence of defects in vesicle acidification and vesicle content. In mice, mosaic KO of VPS50 in the hippocampus altered synaptic transmission and plasticity and generated robust cognitive impairments.
Conclusions
We propose that VPS50 functions as an accessory protein to aid the recruitment of the V-ATPase V1 domain to synaptic vesicles and in that way plays a crucial role in controlling synaptic vesicle acidification. Understanding the mechanisms controlling behaviors and synaptic function in ASD-associated mutations is pivotal for the development of targeted interventions, which may open new avenues for therapeutic strategies aimed at ASD and related conditions.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Howard Hughes Medical Institute
McGovern Institute for Brain Research at MIT
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DOI of Published Version
https://doi.org/10.1186/s12915-024-01940-y
