Noninvasive optical inhibition with a red-shifted microbial rhodopsin
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Lin_Noninvasive optical inhibition.pdf
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Author(s)
Miri, Mitra L.
Busskamp, Volker
Young, Andrew
Ogawa, Masaaki
Ramanlal, Shreshtha B.
Forest, Craig R.
Chow, Brian Y.
Han, Xue
Lin, Yingxi
Roska, Botond
Date Issued
July 2014
Journal
Nature Neuroscience
Publisher
Nature Publishing Group
Citation
Chuong, Amy S, Mitra L Miri, Volker Busskamp, Gillian A C Matthews, Leah C Acker, Andreas T Sorensen, Andrew Young, et al. “Noninvasive Optical Inhibition with a Red-Shifted Microbial Rhodopsin.” Nat Neurosci 17, no. 8 (July 6, 2014): 1123–1129.
Version
Author's final manuscript
Abstract
Optogenetic inhibition of the electrical activity of neurons enables the causal assessment of their contributions to brain functions. Red light penetrates deeper into tissue than other visible wavelengths. We present a red-shifted cruxhalorhodopsin, Jaws, derived from Haloarcula (Halobacterium) salinarum (strain Shark) and engineered to result in red light–induced photocurrents three times those of earlier silencers. Jaws exhibits robust inhibition of sensory-evoked neural activity in the cortex and results in strong light responses when used in retinas of retinitis pigmentosa model mice. We also demonstrate that Jaws can noninvasively mediate transcranial optical inhibition of neurons deep in the brains of awake mice. The noninvasive optogenetic inhibition opened up by Jaws enables a variety of important neuroscience experiments and offers a powerful general-use chloride pump for basic and applied neuroscience.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Massachusetts Institute of Technology. Department of Physics
McGovern Institute for Brain Research at MIT
Picower Institute for Learning and Memory
Program in Media Arts and Sciences (Massachusetts Institute of Technology)
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1038/nn.3752
