Improving electrochemical hybridization assays with restriction enzymes
Name
D3CC06192B.pdf
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865.26 KB
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Checksum (MD5)
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Author(s)
Zhou, Xingcheng
Zamani, Marjon
Austin, Katherine
De Bock, Marieke
Ullola, Joshua Chaj
Rikia, Smah
Furst, Ariel L.
Date Issued
January 29, 2024
Journal
ChemComm
Publisher
Royal Society of Chemistry
Citation
Chem. Commun., 2024,60, 1948-1951
Version
Final published version
Abstract
Nucleic acids in blood are early indicators of disease that could be detected by point-of-care biosensors if sufficiently sensitive and facile sensors existed. Electrochemical hybridization assays are sensitive and specific but are limited to very short nucleic acids. We have developed a restriction enzyme-assisted electrochemical hybridization (REH) assay for improved nucleic acid detection. By incorporating target-specific restriction enzymes, we detect long nucleic acids, with performance dependent on the location of the cut site relative to the electrode surface. Thus, we have further established guidelines for REH design to serve as a generalizable platform for robust electrochemical detection of long nucleic acids.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Center for Environmental Health Sciences
Terms of Use
Creative Commons Attribution-Noncommercial
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1039/D3CC06192B
