Aneuploid yeast strains exhibit defects in cell growth and passage through START
Name
Thorburn-2013-Aneuploid yeast stra.pdf
Size
1.75 MB
Format
Adobe PDF
Checksum (MD5)
5ef22b5e8bcc37e1a6db2ffdd8ca8058
Author(s)
Thorburn, Rebecca Ruth
Gonzalez, Christian
Brar, Gloria A.
Christen, Stefan
Carlile, Thomas M.
Ingolia, Nicholas T.
Sauer, Uwe
Weissman, Jonathan S.
Amon, Angelika B
Date Issued
March 2013
Journal
Molecular Biology of the Cell
Publisher
American Society for Cell Biology
Citation
Thorburn, R. R. et al. “Aneuploid Yeast Strains Exhibit Defects in Cell Growth and Passage Through START.” Molecular Biology of the Cell (2013). ©2013 by The American Society for Cell Biology
Version
Final published version
Abstract
Aneuploidy, a chromosome content that is not a multiple of the haploid karyotype, is associated with reduced fitness in all organisms analyzed to date. In budding yeast aneuploidy causes cell proliferation defects, with many different aneuploid strains exhibiting a delay in G1, a cell cycle stage governed by extracellular cues, growth rate and cell cycle events. Here, we characterize this G1 delay. We show that 10 of 14 aneuploid yeast strains exhibit a growth defect during G1. Furthermore, 10 of 14 aneuploid strains display a cell cycle entry delay that correlates with the size of the additional chromosome. This cell cycle entry delay is due to a delayed accumulation of G1 cyclins that can be suppressed by supplying cells with high levels of a G1 cyclin. Our results indicate that aneuploidy frequently interferes with the ability of cells to grow and, as many other cellular stresses, entry into the cell cycle.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1091/mbc.E12-07-0520
