Random access DNA memory using Boolean search in an archival file storage system
Name
2020.02.05.936369v3.full.pdf
Description
Submitted version
Size
4.63 MB
Format
Adobe PDF
Checksum (MD5)
1b3047a5f0b85aa6301d10d47de6a0be
Author(s)
Banal, James L
Shepherd, Tyson R
Berleant, Joseph
Huang, Hellen
Reyes, Miguel
Ackerman, Cheri M
Blainey, Paul C
Bathe, Mark
Date Issued
2021
Journal
Nature Materials
Publisher
Springer Science and Business Media LLC
Version
Original manuscript
Abstract
DNA is an ultrahigh-density storage medium that could meet exponentially growing worldwide demand for archival data storage if DNA synthesis costs declined sufficiently and if random access of files within exabyte-to-yottabyte-scale DNA data pools were feasible. Here, we demonstrate a path to overcome the second barrier by encapsulating data-encoding DNA file sequences within impervious silica capsules that are surface labelled with single-stranded DNA barcodes. Barcodes are chosen to represent file metadata, enabling selection of sets of files with Boolean logic directly, without use of amplification. We demonstrate random access of image files from a prototypical 2-kilobyte image database using fluorescence sorting with selection sensitivity of one in 106 files, which thereby enables one in 106N selection capability using N optical channels. Our strategy thereby offers a scalable concept for random access of archival files in large-scale molecular datasets.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/s41563-021-01021-3
