Optical visualization and quantification of enzyme activity using dynamic droplet lenses
Name
Optical visualization and quantification of enzyme activity using dynamic droplet lenses
Size
1.11 MB
Format
Unknown
Checksum (MD5)
dae181f1d20dc5895aa78d1bbdd42c47
Author(s)
Zarzar, Lauren D.
Kalow, Julia Ann
He, Xinping
Walish, Joseph John
Swager, Timothy M
Date Issued
March 2017
Journal
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences (U.S.)
Citation
Zarzar, Lauren D., et al. “Optical Visualization and Quantification of Enzyme Activity Using Dynamic Droplet Lenses.” Proceedings of the National Academy of Sciences, vol. 114, no. 15, Apr. 2017, pp. 3821–25. © 2018 National Academy of Sciences
Version
Final published version
Abstract
In this paper, we describe an approach to measuring enzyme activity based on the reconfiguration of complex emulsions. Changes in the morphology of these complex emulsions, driven by enzyme-responsive surfactants, modulate the transmission of light through a sample. Through this method we demonstrate how simple photodetector measurements may be used to monitor enzyme kinetics. This approach is validated by quantitative measurements of enzyme activity for three different classes of enzymes (amylase, lipase, and sulfatase), relying on two distinct mechanisms for coupling droplet morphology to enzyme activity (host–guest interactions with uncaging and molecular cleavage).
MIT Department
Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies
Massachusetts Institute of Technology. Department of Chemistry
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1073/PNAS.1618807114
