Producing and Concentrating Lenti-Cre for Mouse Infections
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nihms621451.pdf
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Author(s)
Gierut, J. J.
Haigis, K. M.
Jacks, Tyler E.
Date Issued
March 2014
Journal
Cold Spring Harbor Protocols
Publisher
Cold Spring Harbor Laboratory Press
Citation
Gierut, J. J. et al. “Producing and Concentrating Lenti-Cre for Mouse Infections.” Cold Spring Harbor Protocols 2014, 3 (March 2014): 304-306 © 2014 Cold Spring Harbor Laboratory Press
Version
Author's final manuscript
Abstract
Lentiviral vectors offer versatility as vehicles for gene delivery. They can transduce a wide range of cell types and integrate into the host genome, which results in long-term expression of the transgene (Cre) both in vitro and in vivo. This protocol describes how lentiviral particles are produced, purified, and concentrated.
MIT Department
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1101/PDB.PROT073437
