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Yeast Display for the Identification of Peptide-MHC Ligands of Immune Receptors

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Author(s)
Huisman, Brooke D
•
Grace, Beth E
•
Holec, Patrick V
•
Birnbaum, Michael E
Date Issued
2022
Journal
Methods Mol Biol
Publisher
Springer US
Citation
Huisman, Brooke D, Grace, Beth E, Holec, Patrick V and Birnbaum, Michael E. 2022. "Yeast Display for the Identification of Peptide-MHC Ligands of Immune Receptors." Methods Mol Biol, 2491.
Abstract
T cells detect peptide antigens presented by major histocompatibility complex (MHC) proteins via their T cell receptor (TCR). The sequence diversity of possible antigens, with trillions of potential peptide-MHC targets, makes it challenging to study, characterize, and manipulate the peptide repertoire of a given TCR. Yeast display has been utilized to study the interactions between peptide-MHCs and T cell receptors to facilitate high-throughput screening of peptide-MHC libraries. Here we present insights on designing and validating a peptide-MHC yeast display construct, designing and constructing peptide libraries, conducting selections, and preparing, processing, and analyzing peptide library sequencing data. Applications for this approach are broad, including characterizing peptide-MHC recognition profiles for a TCR, screening for high-affinity mimotopes of known TCR-binding peptides, and identifying natural ligands of TCRs from expanded T cells.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
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DOI of Published Version
https://doi.org/10.1007/978-1-0716-2285-8_15
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