Apatite-Polymer Composite Particles for Controlled Delivery of BMP-2: In Vitro Release and Cellular Response
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MEBCS001.pdf
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147.89 KB
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18bb6c5786dc6755543dc898c97763c8
Author(s)
Yong, Tseh-Hwan
Ying, Jackie Y.
Date Issued
January 2005
Series/Report no.
Molecular Engineering of Biological and Chemical Systems (MEBCS);
Abstract
Bone morphogenetic protein-2 (BMP-2) has the ability to induce osteoblast differentiation of
undifferentiated cells, resulting in the healing of skeletal defects when delivered with a suitable carrier. We have applied a versatile delivery platform comprising a novel
composite of two biomaterials with proven track records – apatite and poly(lactic-co-glycolic acid)
(PLGA) – to the delivery of BMP-2. Sustained release of this growth factor was tuned with variables that affect polymer degradation and/or apatite dissolution, such as polymer molecular weight, polymer composition, apatite loading, and apatite particle size. The effect of released BMP-2 on C3H10T1/2 murine pluripotent mesenchymal cells was assessed by tracking the expression of osteoblastic makers, alkaline phosphatase (ALP) and osteocalcin. Release media collected over 100 days induced elevated ALP activity in C3H10T1/2 cells. The expression of osteocalcin was
also upregulated significantly. These results demonstrated the potential of apatite-PLGA composite
particles for releasing protein in bioactive form over extended periods of time.
Subjects
Composite
apatite
PLGA
bone morphogenetic protein
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